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|Section2= |Section3= }} Ponceau S, Acid Red 112, or C.I. 27195 is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting), as well as on cellulose acetate membranes.〔 A Ponceau S stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on Western blots for blot-sequencing. It is also easily reversed with water washes, facilitating subsequent immunological detection.〔 The stain can be completely removed from the protein bands by continued washing.〔 Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid.〔(【引用サイトリンク】url=http://www.sigmaaldrich.com/life-science/proteomics/protein-electrophoresis/more-protein-staining-reagents.html )〕 ==See also== *Western blotting *Coomassie Brilliant Blue *Ponceau family of Azo dyes 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Ponceau S」の詳細全文を読む スポンサード リンク
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